產品概述
成熟的腦源性神經生長因子(BDNF，brain-derivedneurotrophicfactor)，是一個分子量為13KD，由119個氨基酸殘基組成的非糖基化多肽。BDNF在氨基酸水平與NGF有52%同源性，在體液中是以非共價連接的同型二聚體。能夠表達BDNF的細胞有成纖維細胞，腦星型膠質細胞，神經細胞，巨核細胞，血小板和平滑肌細胞。在神經系統發育過程中，BDNF可誘導神經細胞的分化，成熟，遷移和觸突的形成。在成體，BDNF最主要的作用之一是神經保護作用，可有效防止前腦神經細胞遭受化學破壞而有效減少神經細胞的死亡。
產品原理
本實驗采用雙抗體夾心ELISA法。抗大鼠BDNF單抗包被于酶標板上，同時加入標本、標準品和生物素化的抗大鼠BDNF抗體，標本、標準品中的BDNF會與加入于孔中的生物素化的抗大鼠BDNF抗體及包被于酶標板上的單抗結合，形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有BDNF，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值，BDNF濃度與OD450值之間呈正相關，可通過繪制標準曲線求出標本中BDNF濃度。
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