本實(shí)驗(yàn)采用雙抗體夾心ELISA法。抗小鼠IgE抗體包被于酶標(biāo)板上，加入標(biāo)準(zhǔn)品和待測(cè)標(biāo)本，標(biāo)準(zhǔn)品和標(biāo)本中的IgE會(huì)與包被于酶標(biāo)板上的抗體結(jié)合，形成免疫復(fù)合物，游離的成分被洗去；加入生物素化的抗小鼠IgE抗體和辣根過(guò)氧化物酶標(biāo)記的親合素，抗小鼠IgE抗體與被捕獲的標(biāo)準(zhǔn)品及待測(cè)標(biāo)本結(jié)合，生物素與親和素特異性結(jié)合，游離的成分被洗去。加入顯色底物（顯色劑），若反應(yīng)孔中有IgE，辣根過(guò)氧化物酶會(huì)使無(wú)色的顯色劑變藍(lán)，加終止液變黃。在450nm處測(cè)OD值，IgE濃度與OD₄₅₀值之間呈正相關(guān)，可通過(guò)繪制標(biāo)準(zhǔn)曲線(xiàn)求出標(biāo)本中IgE濃度。

產(chǎn)品性能

1. 靈敏度：最小可測(cè)小鼠IgE達(dá)2ng/ml。

2.重復(fù)性：板內(nèi)、板間變異系數(shù)均<10%。

產(chǎn)品組分及儲(chǔ)存條件

試驗(yàn)所需自備儀器和試劑:

1. 酶標(biāo)儀(450nm檢測(cè)波長(zhǎng)濾光片，570nm或630nm校正波長(zhǎng)濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、坐標(biāo)紙。

• MOUSE IL-5 ELISA KIT（EM019-96）
• MOUSE Insulin ELISA KIT（EM017-96）
• MOUSE IL-13 ELISA KIT（EM016-96）

• ELISA kit 產(chǎn)品手冊(cè)2019點(diǎn)擊下載

Zhang ZY et al. MitochondrialDNA-LL-37Complex Promotes Atherosclerosis by Escaping from AutophagicRecognition.Immunity, 2015. Read more (PubMed :26680206)

Wang H et al. Loss ofYTHDF2-mediatedm6A-dependent mRNA clearance facilitates hematopoietic stem cellregeneration. CellResearch, 2018. Read more (PubMed :30150673)

Zhang SY et al.Adipocyte-derivedLysophosphatidylcholine Activates Adipocyte and Adipose TissueMacrophage Nod-LikeReceptor Protein 3 Inflammasomes MediatingHomocysteine-Induced InsulinResistance.EBioMedicine, 2018. Read more (Pubmed : 29735414)

He W et al. Vitamin B5 ReducesBacterialGrowth via Regulating Innate Immunity and Adaptive Immunity in MiceInfectedwith Mycobacterium tuberculosis. Front Immunol, 2018. Read more (Pubmed :29535733)

Teng J et al. High-sensitiveimmunosensingof protein biomarker based on interfacial recognition-inducedhomogeneousexponential transcription.Anal Chim Acta, 2019. Read More (PubMed : 31047141)

He MC et al. Paricalcitolalleviateslipopolysaccharide-induced depressive-like behavior by suppressinghypothalamicmicroglia activation and neuroinflammation.  BiochemPharmacol, 2019. Read More (Pubmed :30703351)

Su R et al. A NovelImmunosuppressor,(5R)-5-Hydroxytriptolide, Alleviates Movement Disorder andNeuroinflammation ina 6-OHDA Hemiparkinsonian Rat Model. Aging and Disease,2017. Read More (PubMed: 28203480)