產品概述
細胞間粘附分子-1（Intercellular adhesion molecule-1，ICAM-1），也叫做CD54，是一個普遍存在的跨膜糖蛋白，在白細胞的遷移和激活中起著非常重要的作用。可溶的ICAM-1被報道出現在血清、腦脊髓液、尿液和支氣管肺泡的洗出液中。在這些液體中 sICAM-1（Soluble ICAM-1）量的提高與心血管疾病、II型糖尿病、器官移植失功、氧化劑應激、腹部脂肪聚集、高血壓、肝病和一些惡性腫瘤密切相關。sICAM-1促進血管生成并且可以作為血管內皮細胞激活和受損的一個指標。sICAM-1也可以作為跨膜ICAM-1介導活性的抑制劑，比如抑制單核細胞粘附到激活的內皮細胞，抑制腫瘤細胞對NK細胞介導的裂解的敏感性。
產品原理
本實驗采用雙抗體夾心ELISA法。抗人ICAM-1單抗包被于酶標板上，同時加入標本、標準品和生物素化的抗人ICAM-1抗體，標本、標準品中的ICAM-1會與加入于孔中的生物素化的抗人ICAM-1抗體及包被于酶標板上的單抗結合，形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有ICAM-1，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值， ICAM-1濃度與OD450值之間呈正相關，可通過繪制標準曲線求出標本中ICAM-1濃度。
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