產品概述

白介素 2（IL-2）是通過結合多分子細胞受體復合物發揮生物活力的。多年來，這個受體復合物被認為是由IL-2Rα與IL-2β兩個糖蛋白鏈組成的，兩個亞基相互結合在一起形成一個高親和力受體來傳遞 IL-2信號的。IL-2Rα（也稱為 Tac 抗原或 CD25），是一個分子量為55kD跨膜糖蛋白，有351個氨基酸組成。IL-2β在1989 年被克隆出來，它是個由575個氨基酸組成的跨膜糖蛋白，分子量為75kD，其中有286個氨基酸位于胞漿內，更顯著地參與了細胞信號傳導作用。常認為它是IL-2一種弱抑制劑。在任何情況下，體液中增高的可溶性IL-2Rα常伴隨這T細胞與B細胞的增加與免疫系統的激活。

產品原理

本實驗采用雙抗體夾心ELISA法。抗人IL-2 sRα 單抗包被于酶標板上，標本和標準品中的IL-2 sRα 會與單抗結合形成免疫復合物，游離的成分被洗去；加入生物素化的抗人IL-2 sRα 抗體，生物素化抗人IL-2 sRα 抗體與酶標板上結合的標準品或樣本中的IL-2 sRα 結合而形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有IL-2 sRα，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值，IL-2 sRα 濃度與OD₄₅₀值之間呈正相關，可通過繪制標準曲線求出標本中IL-2 sRα 濃度。

產品性能

1. 靈敏度：最小可測人IL-2 sRα達15pg/ml 。

2. 重復性：板內、板間變異系數均<10%。

產品組分及儲存條件

試驗所需自備儀器和試劑:

1. 酶標儀(450nm檢測波長濾光片，570nm或630nm校正波長濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、坐標紙。

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