產品概述
白細胞介素18（IL-18）是分子量為18KD的細胞因子，最早發現在中毒性休克中，作為共刺激因子以產生干擾素γ（IFN-γ）。IL-18的生物學功能與IL-12非常相似。IL-18以沒有信號肽，分子量為24KD 的前體形式被合成；有活性、成熟的IL-18必須通過切割前體產成。白細胞介素1β轉換酶（ICE, caspase-1）在天冬氨酸的p1位置切開IL-18前體，產生具有生物活性的，可以從細胞內分泌成熟的IL-18。文獻報道，IL-18主要來源于活化的巨噬細胞，樹突狀細胞，Kupffer細胞，角質形成細胞，腸上皮細胞，成骨細胞，腎上腺皮質細胞和小鼠間腦等。
產品原理
本實驗采用雙抗體夾心ELISA法?？谷薎L-18單抗包被于酶標板上，同時加入標本、標準品和生物素化的抗人IL-18抗體，標本、標準品中的IL-18會與加入于孔中的生物素化的抗人IL-18抗體及包被于酶標板上的單抗結合，形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有IL-18，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值， IL-18濃度與OD450值之間呈正相關，可通過繪制標準曲線求出標本中IL-18濃度。
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