本實驗采用雙抗體夾心ELISA法。抗人DKK-1單抗包被于酶標板上，標本和標準品中的DKK-1會與單抗結合形成免疫復合物，游離的成分被洗去；加入生物素化的抗人DKK-1抗體，生物素化抗人DKK-1抗體與酶標板上結合的標準品或樣本中的DKK-1結合而形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有DKK-1，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值， DKK-1濃度與OD₄₅₀值之間呈正相關，可通過繪制標準曲線求出標本中DKK-1濃度。

產品性能

1. 靈敏度：最小可測人DKK-1達30pg/ml 。

2. 重復性：板內、板間變異系數均<10%。

產品組分及儲存條件

試驗所需自備儀器和試劑:

1. 酶標儀(450nm檢測波長濾光片，570nm或630nm校正波長濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、坐標紙。

• HUMAN IL-3 ELISA KIT（EH058-96）
• HUMAN IL-15 ELISA KIT（EH057-96）
• HUMAN MIP-1α ELISA KIT（EH055-96）

• ELISA kit 產品手冊2019點擊下載

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