本實驗采用雙抗體夾心ELISA法。抗HIV-1 Gag p24單抗包被于酶標(biāo)板上，同時加入標(biāo)本、標(biāo)準(zhǔn)品和生物素化的抗HIV-1 Gag p24抗體，標(biāo)本、標(biāo)準(zhǔn)品中的HIV-1 Gag p24會與加入于孔中的生物素化的抗HIV-1 Gag p24抗體及包被于酶標(biāo)板上的單抗結(jié)合，形成免疫復(fù)合物，游離的成分被洗去；加入辣根過氧化物酶標(biāo)記的親合素，親合素與生物素特異性結(jié)合，游離的成分被洗去。加入顯色底物（顯色劑），若反應(yīng)孔中有HIV-1 Gag p24，辣根過氧化物酶會使無色的顯色劑變藍(lán)，加終止液變黃。在450nm 處測OD值，HIV-1 Gag p24 濃度與OD450值之間呈正相關(guān)，可通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中HIV-1 Gag p24度。

產(chǎn)品性能

1. 靈敏度：最小可測人HIV-1 Gag p24達(dá)10pg/ml 。

2. 重復(fù)性：板內(nèi)、板間變異系數(shù)均<10%。

產(chǎn)品組分及儲存條件

試驗所需自備儀器和試劑:

1. 酶標(biāo)儀(450nm檢測波長濾光片，570nm或630nm校正波長濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、微量振蕩器、坐標(biāo)紙。

• HUMAN ADAMTS1 ELISA KIT（EH052-96）
• HUMAN IL-17R ELISA KIT（EH051-96）
• HUMAN MMP-2 ELISA KIT（EH048-96）

• ELISA kit 產(chǎn)品手冊2019點擊下載

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